Serotonin does not increase the EPSP amplitude and IP3 does not decrease the EPSP amplitude in Orconectes neuromuscular junction

  • Yufei Wang Grinnell College
  • Danica Bojovic Grinnell College
  • Lysimachos Papoutsis Grinnell College

Abstract

Serotonin (5-HT) is a neuromodulator known to facilitate neurotransmission in the neuromuscular junction (NMJ). It binds to presynaptic receptors, and activates G-proteins which then leads to the activation of second messenger molecule called IP3. Our goal was to investigate whether IP3 causes the release of internal Ca2+, leading to facilitation. We investigated the effects of 5-HT and the role of IP3 in crayfish (Orconectes) synaptic transmission. We hypothesized that 5-HT would increase the excitatory post-synaptic potential (EPSP) amplitude in crayfish (Orconectes) NMJ. Furthermore, we hypothesized that the inhibition of IP3 receptors using 2-APB would block the effects of 5-HT and therefore prevent facilitation. In our experiment, 5-HT led to EPSP decreasing by 26%. The addition of 2-APB with 5-HT solution led to 61% higher EPSP amplitude compared to control (saline solution). However, our t-test (p>.05) showed that these differences were not significant. Thus, our results show that 5-HT and 2-APB do not affect the EPSP amplitude in the crayfish (Orconectes) NMJ.
Published
2015-12-21
How to Cite
WANG, Yufei; BOJOVIC, Danica; PAPOUTSIS, Lysimachos. Serotonin does not increase the EPSP amplitude and IP3 does not decrease the EPSP amplitude in Orconectes neuromuscular junction. Pioneering Neuroscience, [S.l.], v. 15, p. 37-40, dec. 2015. Available at: <https://ojs.grinnell.edu/index.php/pnsj/article/view/296>. Date accessed: 18 nov. 2017.
Section
Articles