Inhibiting reverse-mode operation of the Na+/Ca2+ exchanger does not affect long-term facilitation in crayfish neuromuscular junction

  • Sophie Banegas Grinnell College
  • Savanna Biedermann Grinnell College
  • Ashley Wehrenberg Grinnell College

Abstract

High frequency stimulation of a crayfish neuron results in a long-term facilitation (LTF) of synaptic transmission of neurotransmitters across the neuromuscular junction. After LTF is induced, the Na+/Ca2+ exchangers begin working in a reverse mode, expelling three Na+ ions and admitting one Ca2+ ion into the cell. We hypothesized that inhibiting the reverse mode of the Na+/Ca2+ exchanger with the chemical SN-6 would result in a decrease in postsynaptic EPSPs after low frequency (10 Hz) stimulation for a minute following high frequency stimulation (20 Hz) for 10 minutes. We then recorded EPSPs before and after both the long and short term LTF-inducing tetani. The difference in means of our EPSPs showed had no statistical significance (p>0.05).
Published
2015-12-21
How to Cite
BANEGAS, Sophie; BIEDERMANN, Savanna; WEHRENBERG, Ashley. Inhibiting reverse-mode operation of the Na+/Ca2+ exchanger does not affect long-term facilitation in crayfish neuromuscular junction. Pioneering Neuroscience, [S.l.], v. 15, p. 25-29, dec. 2015. Available at: <https://ojs.grinnell.edu/index.php/pnsj/article/view/295>. Date accessed: 21 sep. 2017.
Section
Articles