TY  - JOUR
AU  - Barbey, Christopher W.
AU  - Doers, Matthew E.
PY  - 2010
TI  -  Voltage Dependency of Neurotransmitter Release at the Crayfish Neuromuscular Junction
JF  - Pioneering Neuroscience; Vol 10 (2010)
KW  - 
N2  - To test the new hypothesis that a calcium independent mechanism is required for neurotransmitter release, we measured excitatory post-synaptic potentials (EPSPs) at the neuromuscular junction of the crayfish tail extensor muscle. Stimulation of the nerve was accomplished with a suction electrode and measurements were made with a micropipette placed in a post-synaptic muscle cell. The tail was bathed in Co 2+  to effectively eliminate EPSPs. Next, the calcium ionophore A23187 was added to raise the intracellular concentration of Ca 2+ . Upon addition of A23187 a small recovery of EPSP was observed in dilute cobalt concentrations but this was reversed using a higher concentration of Co 2+ . Our results neither confirm nor deny either theory.
UR  - https://ojs.grinnell.edu/index.php/pnsj/article/view/83