TY  - JOUR
AU  - Morley, Drew
AU  - Shriver, Kirsten
AU  - Zhang, Xingjie
PY  - 2010
TI  -  Nitric Oxide Mediates the Effect of DF 2  on EPSP Amplitude
JF  - Pioneering Neuroscience; Vol 10 (2010)
KW  - 
N2  - This experiment looked at the role of nitric oxide in the mechanism by which the peptide DF 2  increases neurotransmitter release at the crayfish neuromuscular junction. We hypothesized that if DF 2  increases neurotransmitter release through a pathway involving nitric oxide as a retrograde signal, then when L-NAME, an inhibitor of nitric oxide synthesis, was applied, DF 2  will not increase neurotransmitter release. To test this we exposed crayfish superficial dorsal extensor muscles to DF 2 , which was amplified with IBMX. We then submerged the preparation into saline solution that contained DF 2 , IBMX, and L-NAME. Neurotransmitter release was measured by the amplitude of EPSP traces recorded from the postsynaptic muscle cells via intracellular recording. Our data shows an increase in neurotransmitter release after exposure to DF 2  and IBMX, as well as a decrease in neurotransmitter release after the addition of L-NAME, a trend that supports our hypothesis. Further testing is needed to draw conclusions about our hypothesis.
UR  - https://ojs.grinnell.edu/index.php/pnsj/article/view/78