TY  - JOUR
AU  - Gorski, Andrew
AU  - Hainline, Margaret
AU  - Pennington, Peter
PY  - 2013/01/25
TI  -  Morphine Sulfate and Naloxone Hydrochloride Decrease EPSP Amplitude at the Crayfish Neuromuscular Junction
JF  - Pioneering Neuroscience; Vol 2 (2000)
KW  - 
N2  - We tested for the presence of opioid receptors at the crayfish neuromuscular junction by measuring the effects of morphine and naloxone on EPSP amplitudes. If opioid receptors were present, we expected morphine to decrease EPSP amplitudes and naloxone to reverse morphine’s effect. Our results show that morphine decreased EPSP amplitude, but naloxone failed to reverse morphine's effect. Naloxone applied exclusively, unexpectedly decreased EPSP amplitudes. Our results did not support the existence of known opioid receptors. However, our results suggest that morphine does have an effect at this junction. This effect could be due to nonspecific binding, unintended chemical interference, or the existence of an undiscovered opioid receptor. Our results could lead to future experiments leading to the discovery of an unknown opioid receptor or experiments regarding how morphine elicits a decrease in EPSPs in the absence of opioid receptors.
UR  - https://ojs.grinnell.edu/index.php/pnsj/article/view/177