TY  - JOUR
AU  - Bardelson, Catherine L.
AU  - Carenen, Bonnie K.
AU  - Henry, Mark G.
PY  - 2003
TI  -  Chelating Intracellular Ca 2+  Increases the Time Needed for the Recovery of the Vesicle Pool after High-frequency Stimulation
JF  - Pioneering Neuroscience; Vol 4 (2002)
KW  - 
N2  - To study the effects of calcium on the readily releasable vesicle pool in presynaptic cells of the crayfish neuromuscular junction, we used the drug EGTA-AM, an intracellular Ca 2+  chelator. We measured the effects of Ca 2+  on the cells' abilities to transmit nerve impulses by recording excitatory postsynaptic potentials (EPSPs), an indicator of vesicle release, both before and after a period of high-frequency stimulation. A cell’s vesicle pool was considered replenished when it regained its original EPSP amplitude. Our research shows that buffering intracellular Ca 2+  decreases the cells' abilities to replenish vesicle supplies; it took longer for cells to recover from high-frequency stimulation and they did not appear to recover fully, even after several minutes.
UR  - https://ojs.grinnell.edu/index.php/pnsj/article/view/156