%A Bardelson, Catherine L.
%A Carenen, Bonnie K.
%A Henry, Mark G.
%D 2003
%T  Chelating Intracellular Ca 2+  Increases the Time Needed for the Recovery of the Vesicle Pool after High-frequency Stimulation
%K 
%X To study the effects of calcium on the readily releasable vesicle pool in presynaptic cells of the crayfish neuromuscular junction, we used the drug EGTA-AM, an intracellular Ca 2+  chelator. We measured the effects of Ca 2+  on the cells' abilities to transmit nerve impulses by recording excitatory postsynaptic potentials (EPSPs), an indicator of vesicle release, both before and after a period of high-frequency stimulation. A cell’s vesicle pool was considered replenished when it regained its original EPSP amplitude. Our research shows that buffering intracellular Ca 2+  decreases the cells' abilities to replenish vesicle supplies; it took longer for cells to recover from high-frequency stimulation and they did not appear to recover fully, even after several minutes.
%U https://ojs.grinnell.edu/index.php/pnsj/article/view/156
%J Pioneering Neuroscience
%0 Journal Article
%P 13-15%V 4
%8 2003-01-01